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primary human bronchial epithelial cells hbepc  (PromoCell)


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    PromoCell primary human bronchial epithelial cells hbepc
    Primary Human Bronchial Epithelial Cells Hbepc, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human bronchial epithelial cells hbepc/product/PromoCell
    Average 95 stars, based on 123 article reviews
    primary human bronchial epithelial cells hbepc - by Bioz Stars, 2026-03
    95/100 stars

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    Effect of TQ-ox on apoptosis in HGEpiCs and AGS cells. Statistical significance was analyzed using one-way analysis of variance with Tukey's post hoc test. **P<0.01 and ***P<0.001 vs. control. Representative fluorescence microscopy images of acridine orange/ethidium bromide-stained cells showing live (green) and apoptotic (red/orange) cells in the control and 40 µM TQ-ox treated AGS groups. A total of ~50 cells were analyzed per condition. Scale bar, 100 µm. HGEpiC, human gastric epithelial cell; TQ-ox, thymoquinone-oxime.

    Journal: Oncology Letters

    Article Title: Cytotoxic, apoptotic and genotoxic effects of thymoquinone-oxime derivative on gastric cancer cells: An in vitro study

    doi: 10.3892/ol.2026.15462

    Figure Lengend Snippet: Effect of TQ-ox on apoptosis in HGEpiCs and AGS cells. Statistical significance was analyzed using one-way analysis of variance with Tukey's post hoc test. **P<0.01 and ***P<0.001 vs. control. Representative fluorescence microscopy images of acridine orange/ethidium bromide-stained cells showing live (green) and apoptotic (red/orange) cells in the control and 40 µM TQ-ox treated AGS groups. A total of ~50 cells were analyzed per condition. Scale bar, 100 µm. HGEpiC, human gastric epithelial cell; TQ-ox, thymoquinone-oxime.

    Article Snippet: Primary HGEpiCs were obtained from Innoprot (cat. no. P10778).

    Techniques: Control, Fluorescence, Microscopy, Staining

    Effect of TQ-ox on DNA damage in HGEpiCs and AGS cells following 24 h of TQ-ox treatment (5–40 µM). Statistical significance was analyzed using one-way analysis of variance with Tukey's post hoc test. **P<0.01 and ***P<0.001 vs. control. Representative fluorescence microscopy images showing nuclei (control) and comet tails (40 µM TQ-ox-treated cells), indicating fragmented DNA. A total of ~50 cells were analyzed per condition. Scale bar, 200 µm. HGEpiC, human gastric epithelial cell; TQ-ox, thymoquinone-oxime.

    Journal: Oncology Letters

    Article Title: Cytotoxic, apoptotic and genotoxic effects of thymoquinone-oxime derivative on gastric cancer cells: An in vitro study

    doi: 10.3892/ol.2026.15462

    Figure Lengend Snippet: Effect of TQ-ox on DNA damage in HGEpiCs and AGS cells following 24 h of TQ-ox treatment (5–40 µM). Statistical significance was analyzed using one-way analysis of variance with Tukey's post hoc test. **P<0.01 and ***P<0.001 vs. control. Representative fluorescence microscopy images showing nuclei (control) and comet tails (40 µM TQ-ox-treated cells), indicating fragmented DNA. A total of ~50 cells were analyzed per condition. Scale bar, 200 µm. HGEpiC, human gastric epithelial cell; TQ-ox, thymoquinone-oxime.

    Article Snippet: Primary HGEpiCs were obtained from Innoprot (cat. no. P10778).

    Techniques: Control, Fluorescence, Microscopy

    Core Hippo kinase regulation of CSPG4 in TcdB exposed pericytes . A , pericytes were exposed to 1 ng/ml of TcdB2 for 24 h and colonic epithelial cells were exposed to 10 ng/ml of TcdB2 for 24 h. Multiple wells from each treatment were combined for RNA isolation. RT-qPCR was used to quantify CSPG4 transcripts. RT-qPCR data are presented as mean (n = 3) ± S.D. with each data point as a technical replicate. ∗ p < 0.05 determined by Student’s t test. B , Immunoblots from protein lysates acquired from pericytes exposed for 24 h to 1 ng/ml of TcdB. C , immunoblots from protein lysates obtained from pericytes or HeLa cells exposed for 24 h to 1 ng/ml of TcdB. Phosphorylated LATS1 (pLATS1) was detected with an antibody recognizing phosphorylation at Thr 1079. D , densitometry analysis of pLATS1 immunoblots. Relative band density is presented as mean (n = 2) ± S.D. with each data point as a technical replicate. ∗ p < 0.05 determined by Student’s t test. E , immunoblots from protein lysates acquired from pericyte or HeLa cells exposed for 24 h to 1 ng/ml of TcdB in the presence or absence of 10 μM XMU-MP-1. F , immunoblots from protein lysates taken from HeLa cells exposed for 24 h to 1 ng/ml of TcdB with and without of 30 μM TRULI.

    Journal: The Journal of Biological Chemistry

    Article Title: Clostridioides difficile TcdB induces expression of its receptor (CSPG4) through a noncanonical Hippo signaling mechanism

    doi: 10.1016/j.jbc.2026.111137

    Figure Lengend Snippet: Core Hippo kinase regulation of CSPG4 in TcdB exposed pericytes . A , pericytes were exposed to 1 ng/ml of TcdB2 for 24 h and colonic epithelial cells were exposed to 10 ng/ml of TcdB2 for 24 h. Multiple wells from each treatment were combined for RNA isolation. RT-qPCR was used to quantify CSPG4 transcripts. RT-qPCR data are presented as mean (n = 3) ± S.D. with each data point as a technical replicate. ∗ p < 0.05 determined by Student’s t test. B , Immunoblots from protein lysates acquired from pericytes exposed for 24 h to 1 ng/ml of TcdB. C , immunoblots from protein lysates obtained from pericytes or HeLa cells exposed for 24 h to 1 ng/ml of TcdB. Phosphorylated LATS1 (pLATS1) was detected with an antibody recognizing phosphorylation at Thr 1079. D , densitometry analysis of pLATS1 immunoblots. Relative band density is presented as mean (n = 2) ± S.D. with each data point as a technical replicate. ∗ p < 0.05 determined by Student’s t test. E , immunoblots from protein lysates acquired from pericyte or HeLa cells exposed for 24 h to 1 ng/ml of TcdB in the presence or absence of 10 μM XMU-MP-1. F , immunoblots from protein lysates taken from HeLa cells exposed for 24 h to 1 ng/ml of TcdB with and without of 30 μM TRULI.

    Article Snippet: Primary human colonic epithelial cells (36,037–08) were obtained from Celprogen and were cultured using the protocol provided by Celprogen.

    Techniques: Isolation, Quantitative RT-PCR, Western Blot, Phospho-proteomics